A Proteomic Network for Symbiotic Nitrogen Fixation Efficiency in Bradyrhizobium elkanii

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Draft genome sequence of the nitrogen-fixing symbiotic bacterium Bradyrhizobium elkanii 587.

The draft sequence of the genome of Bradyrhizobium elkanii 587 is presented. This was obtained using Illumina Next-Gen DNA sequencing combined with Sanger sequencing. Genes for the pathways involved in biological nitrogen fixation (the nif gene cluster), nod genes including nodABC, and genes for the type III protein secretion system (T3SS) are present.

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Symbiotic Nitrogen Fixation.

Biosphere nitrogen is subjected to rapid turnover, and because it is eventually lost as nitrogen into the atmosphere, its maintenance requires continuous replenishment with reduced nitrogen from atmospheric nitrogen. Biological reduction of nitrogen to ammonia can be performed only by some prokaryotes and is a highly oxygen-sensitive process. The most efficient nitrogen fixers establish a symbi...

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Bradyrhizobium japonicum cytochrome c550 is required for nitrate respiration but not for symbiotic nitrogen fixation.

Bradyrhizobium japonicum possesses three soluble c-type cytochromes, c550, c552, and c555. The genes for cytochromes c552 (cycB) and c555 (cycC) were characterized previously. Here we report the cloning, sequencing, and mutational analysis of the cytochrome c550 gene (cycA). A B. japonicum mutant with an insertion in cycA failed to synthesize a 12-kDa c-type cytochrome. This protein was detecta...

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Isocitrate dehydrogenase of Bradyrhizobium japonicum is not required for symbiotic nitrogen fixation with soybean.

A mutant strain of Bradyrhizobium japonicum USDA110 lacking isocitrate dehydrogenase activity was created to determine whether this enzyme was required for symbiotic nitrogen fixation with soybean (Glycine max cv. Williams 82). The isocitrate dehydrogenase mutant, strain 5051, was constructed by insertion of a streptomycin resistance gene cassette. The mutant was devoid of isocitrate dehydrogen...

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ژورنال

عنوان ژورنال: Molecular Plant-Microbe Interactions®

سال: 2018

ISSN: 0894-0282,1943-7706

DOI: 10.1094/mpmi-10-17-0243-r